Anti-IDH2 Polyclonal Antibody (HW514014)

Name: Anti-IDH2 Polyclonal Antibody
Brand: abinScience
SKU: HW514014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW514014

Description

Anti-IDH2 Polyclonal Antibody (HW514014) is a rabbit polyclonal antibody detecting IDH2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IDH2 (Ala40-Gln452).

Target

Isocitrate dehydrogenase [NADP], mitochondrial, IDH2, IDH, ICD-M, NADP(+)-specific ICDH, IDP, Oxalosuccinate decarboxylase

Purification

Purified by antigen affinity column.

Accession

P48735

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) is a ~50 kDa protein. Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

1. Yan, H. et al. (2009) The New England journal of medicine 360, 765-73. PMID: 19228619

2. Yu, W. et al. (2012) The Journal of biological chemistry 287, 14078-86. PMID: 22416140

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IDH2 polyclonal antibody at 1ug/mL on recombinant IDH2 (Catalog No. HW514012).

References

Datasheet

Anti-IDH2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IDH2 Polyclonal Antibody (HW514014)

Overview

Images

References

Datasheet

SDS

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