Anti-IDH3G Polyclonal Antibody (HA679014)

Name: Anti-IDH3G Polyclonal Antibody
Brand: abinScience
SKU: HA679014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA679014

Description

Anti-IDH3G Polyclonal Antibody (HA679014) is a rabbit polyclonal antibody detecting mitochondrial; Isocitric dehydrogenase subunit gamma; NAD(+)-specific ICDH subunit gamma; IDH3G in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human IDH3G (Gly53-Ala393).

Target

Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; Isocitric dehydrogenase subunit gamma; NAD(+)-specific ICDH subunit gamma; IDH3G

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P51553, Q58CP0, P41565, P70404

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial (IDH3G) is a ~42 kDa protein. Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-IDH3G polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Mouse brain lysate
Lane 3: Rat heart lysate
Lane 4: Rat brain lysate

References

Datasheet

Anti-IDH3G Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IDH3G Polyclonal Antibody (HA679014)

Overview

Images

References

Datasheet

SDS