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Anti-IFI30 Polyclonal Antibody (MY158014)

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Overview
Catalog No.MY158014
Description
Anti-IFI30 Polyclonal Antibody (MY158014) is a rabbit polyclonal antibody detecting Gamma-interferon-inducible lysosomal thiol reductase; 1.8.-.-; Gamma-interferon-inducible protein IP-30; Lysosomal thiol reductase IP30; Ifi30; Gilt; Ip30 in ELISA, IHC, WB. Suitable for Rat and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant mouse IFI30 (Val59-Lys248).
Target Gamma-interferon-inducible lysosomal thiol reductase; 1.8.-.-; Gamma-interferon-inducible protein IP-30; Lysosomal thiol reductase IP30; Ifi30; Gilt; Ip30
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9ESY9, Q499T2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Gamma-interferon-inducible lysosomal thiol reductase (IFI30) is a ~27 kDa protein. Lysosomal thiol reductase that catalyzes protein disulfide bonds reduction. Plays an important role in antigen processing and presentation, of namely both major histocompatibility complex (MHC) class I- and class II-restricted antigen by reducing the disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation. Shares with thioredoxin a reduction mechanism in which the N-terminal cysteine thiol group in the CXXC active site motif initiates a nucleophilic attack on the substrate disulfide bond,resulting in the formation of a mixed disulfide-linked enzyme-substrate intermediate (Probable). Subsequent intramolecular attack by the second cysteine thiol group enables the release of the reduced substrate and oxidized enzyme (Probable). Lysosomal cysteine is a physiological reducing agent that is capable of reducing IFI30, so that it can catalyzes the next reaction.

1. Maric, M. et al. (2001) Science (New York, N.Y.) 294, 1361-5. PMID: 11701933
2. Singh, R. et al. (2010) Science (New York, N.Y.) 328, 1394-8. PMID: 20538950
Note For research use only
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Datasheet
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Formula
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Anti-IFI30 Polyclonal Antibody

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