Anti-IL1A/IL1F1 Polyclonal Antibody (HF802014)

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Spec:

50ug
100ug

Number:

Overview
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Datasheet
SDS

Overview

Catalog No.

HF802014

Description

Anti-IL1A/IL1F1 Polyclonal Antibody (HF802014) is a rabbit polyclonal antibody detecting IL1F1 in ELISA, IHC, WB. Suitable for Human, Macaca fascicularis, and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Macaca fascicularis, Macaca mulatta

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL1A/IL1F1 (Ser113-Ala271).

Target

IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha

Purification

Purified by antigen affinity column.

Accession

P01583

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-1 alpha (IL1A/IL1F1) is a ~30 kDa protein. Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage.

1. Cohen, I. et al. (2015) Scientific reports 5, 14756. PMID: 26439902

2. Brikos, C. et al. (2007) Molecular & cellular proteomics : MCP 6, 1551-9. PMID: 17507369

3. Mosley, B. et al. (1987) The Journal of biological chemistry 262, 2941-4. PMID: 2950091

4. Wu, T. et al. (2004) Cytokine 25, 11-20. PMID: 14687581

5. Spiekstra, SW. et al. (2005) Experimental dermatology 14, 109-16. PMID: 15679580

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IL1A/IL1F1 polyclonal antibody at 1ug/mL on recombinant IL1A/IL1F1 (Catalog No. HF802012).

References

Datasheet