Anti-IL1B/IL1F2 Polyclonal Antibody (HF943014)

Name: Anti-IL1B/IL1F2 Polyclonal Antibody
Brand: abinScience
SKU: HF943014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF943014

Description

Anti-IL1B/IL1F2 Polyclonal Antibody (HF943014) is a rabbit polyclonal antibody detecting IL1F2 in ELISA, IHC, WB. Suitable for Human, Cercocebus atys, Macaca fascicularis, Macaca mulatta, and Macaca nemestrina.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL1B/IL1F2 (Ala117-Ser269).

Target

Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta, IL-1β

Purification

Purified by antigen affinity column.

Accession

P01584

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-1 beta (IL1B/IL1F2) is a ~30 kDa protein. Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. IL1B is the therapeutic target of canakinumab (Ilaris).

1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850

2. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819

3. LaRock, CN. et al. (2016) Science immunology 1. PMID: 28331908

4. Van Damme, J. et al. (1985) Nature 314, 266-8. PMID: 3920526

5. Tian, XX. et al. (2021) International endodontic journal 54, 916-925. PMID: 33377178

6. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IL1B/IL1F2 polyclonal antibody at 1ug/mL on recombinant IL1B/IL1F2 (Catalog No. HF943012).

References

Datasheet