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Anti-Influenza A virus RNA polymerase complex Recombinant Nanobody (SAA1427) (VK560013)

Anti-Influenza A virus RNA polymerase complex Recombinant Nanobody (SAA1427)
Anti-Influenza A virus RNA polymerase complex Recombinant Nanobody (SAA1427)
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Overview
Catalog No.VK560013
Description
Anti-Influenza A virus RNA polymerase complex Nanobody (SAA1427) [SAA1427] (VK560013) is a alpaca monoclonal antibody detecting RNA-directed RNA polymerase subunit P2 in ELISA.
Species reactivityInfluenza A virus (H3N2)
ApplicationsELISA
Host speciesAlpaca
IsotypeVHH-8His-Cys-tag
Clone IDSAA1427
Clonality Monoclonal
Target Polymerase acidic protein, 3.1.-.-, RNA-directed RNA polymerase subunit P2, PA
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Accession P03434
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Polymerase acidic protein (RNA polymerase complex) is a ~82 kDa protein. Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Note For research use only.
Images
  • Anti-Influenza A virus RNA polymerase complex Recombinant Nanobody (SAA1427)

    SDS-PAGE

    SDS-PAGE for Anti-Influenza A virus RNA polymerase complex Nanobody (SAA1427)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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