

| Catalog No. | VK560013 | ||||
|---|---|---|---|---|---|
| Description |
Anti-Influenza A virus RNA polymerase complex Nanobody (SAA1427) [SAA1427] (VK560013) is a alpaca monoclonal antibody detecting RNA-directed RNA polymerase subunit P2 in ELISA.
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| Species reactivity | Influenza A virus (H3N2) | ||||
| Applications | ELISA | ||||
| Host species | Alpaca | ||||
| Isotype | VHH-8His-Cys-tag | ||||
| Clone ID | SAA1427 | ||||
| Clonality | Monoclonal | ||||
| Target | Polymerase acidic protein, 3.1.-.-, RNA-directed RNA polymerase subunit P2, PA | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Purification | Purified by Nickel column. | ||||
| Accession | P03434 | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Background | Polymerase acidic protein (RNA polymerase complex) is a ~82 kDa protein. Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. | ||||
| Note | For research use only. |

SDS-PAGE for Anti-Influenza A virus RNA polymerase complex Nanobody (SAA1427)
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