Anti-IRAK4 Polyclonal Antibody (HV737014)

Name: Anti-IRAK4 Polyclonal Antibody
Brand: abinScience
SKU: HV737014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HV737014

Description

Anti-IRAK4 Polyclonal Antibody (HV737014) is a rabbit polyclonal antibody detecting IRAK4 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IRAK4 (Met1-Val200).

Target

Renal carcinoma antigen NY-REN-64, IRAK-4, IRAK4, Interleukin-1 receptor-associated kinase 4

Purification

Purified by antigen affinity column.

Accession

Q9NWZ3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-1 receptor-associated kinase 4 (IRAK4) is a ~51 kDa protein. Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1.

1. Hoarau, C. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 4754-65. PMID: 17878374

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IRAK4 polyclonal antibody at 1ug/mL on recombinant IRAK4 (Catalog No. HV737012).

References

Datasheet

Anti-IRAK4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IRAK4 Polyclonal Antibody (HV737014)

Overview

Images

References

Datasheet

SDS

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