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Anti-IRX4 Polyclonal Antibody (HB510014)

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Overview
Catalog No.HB510014
Description
Anti-IRX4 Polyclonal Antibody (HB510014) is a rabbit polyclonal antibody detecting Iroquois-class homeodomain protein IRX-4; Homeodomain protein IRXA3; Iroquois homeobox protein 4; IRX4; IRXA3 in ELISA, IHC, WB. Suitable for Chicken, Human, Frog, Mouse, and Zebrafish.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human IRX4 (Asn148-Met206).
Target Iroquois-class homeodomain protein IRX-4; Homeodomain protein IRXA3; Iroquois homeobox protein 4; IRX4; IRXA3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9YGS0, P78413, Q90XW6, B7ZRT8, Q9QY61, P78412, P78415, P81067, Q503Z8, O42261, P81068, Q9ER75, P78414, Q6DCQ1, Q2TAQ8, Q9BZI1, Q1LXU5, Q9JKQ4, P78411, Q9YGK8, P54269, Q24248, P81066, Q90XW5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Iroquois-class homeodomain protein IRX-4 (IRX4) is a ~52 kDa protein. Regulates the chamber-specific expression of myosin isoforms by activating the expression of the ventricle myosin heavy chain-1 (Vmhc1) and suppressing the expression of the atrial myosin heavy chain-1 (Amhc1) in the ventricles. May play a critical role in establishing chamber-specific gene expression in the developing heart.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-IRX4 Polyclonal Antibody

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