Please ensure Javascript is enabled for purposes of website accessibility
Contact us Contact Us
EN
Home > Products > Antibodies > Primary antibodies

Anti-ITK Polyclonal Antibody (HB378014)

Datasheet Share product Copy info
Price(USD): --
Spec:
  • 50ug
  • 100ug
Number:
Get quote

  • Overview

  • Images

  • References

  • Datasheet

  • SDS

Overview
Catalog No.HB378014
Description
Anti-ITK Polyclonal Antibody (HB378014) is a rabbit polyclonal antibody detecting Tyrosine-protein kinase ITK/TSK; 2.7.10.2; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk; ITK; EMT; LYK in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human ITK (Glu173-Leu620).
Target Tyrosine-protein kinase ITK/TSK; 2.7.10.2; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk; ITK; EMT; LYK
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q08881, Q03526
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tyrosine-protein kinase ITK/TSK is a ~71 kDa protein. Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates.

1. Perez-Villar, JJ. et al. (2002) Biochemistry 41, 10732-40. PMID: 12186560
2. Grasis, JA. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 3971-6. PMID: 12682224
3. Sela, M. et al. (2011) The EMBO journal 30, 3160-72. PMID: 21725281
Note For research use only
Images
References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: [email protected]

Distributor list

Get a free quote
Anti-ITK Polyclonal Antibody

Quick access

Contact Us

  • +86-27-65523339

  • [email protected]

  • Building C, No. 666, Shen Dun Si Lu, Wuhan, 430206, China

  • linkedin
  • Ins
  • youtube
  • youtube
Copyright © 2026 abinScience. All Rights Reserved. All Products are for Research Use Only