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Anti-KLF13 Polyclonal Antibody (HC082014)

Anti-KLF13 Polyclonal Antibody
Anti-KLF13 Polyclonal Antibody
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Overview
Catalog No.HC082014
Description
Anti-KLF13 Polyclonal Antibody (HC082014) is a rabbit polyclonal antibody detecting KLF13 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant KLF13 (Gly179-Gly255).
Target Krueppel-like factor 13, Basic transcription element-binding protein 3, BTE-binding protein 3, Novel Sp1-like zinc finger transcription factor 1, RANTES factor of late activated T-lymphocytes 1, RFLAT-1, Transcription factor BTEB3, Transcription factor NSLP1, KLF13, BTEB3, NSLP1
Endotoxin level Please contact with the lab for this information.
Observed Molecular Weight 38 kDa
Purification Purified by antigen affinity column.
Accession Q9Y2Y9, Q9JJZ6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Krueppel-like factor 13 (KLF13) is a ~31 kDa protein. Transcription factor that activates expression from GC-rich minimal promoter regions, including genes in the cells of the erythroid lineage. Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES and CCL5 expression in T-cells.

1. Kaczynski, J. et al. (2001) The Journal of biological chemistry 276, 36749-56. PMID: 11477107
2. Huang, B. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 7081-7. PMID: 17513757
Note For research use only
Images
  • Anti-KLF13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-KLF13 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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