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Anti-KLK7 Polyclonal Antibody (HW573014)

Anti-KLK7 Polyclonal Antibody
Anti-KLK7 Polyclonal Antibody
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Overview
Catalog No.HW573014
Description
Anti-KLK7 Polyclonal Antibody (HW573014) is a rabbit polyclonal antibody detecting KLK7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human KLK7 (Glu23-Arg253).
Target hSCCE, Stratum corneum chymotryptic enzyme, PRSS6, Kallikrein-7, Serine protease 6, hK7, KLK7, SCCE
Purification Purified by antigen affinity column.
Accession P49862
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Kallikrein-7 (KLK7) is a ~27 kDa protein. May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

Note For research use only.
Images
  • Anti-KLK7 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-KLK7 polyclonal antibody at 1ug/mL on recombinant KLK7 (Catalog No. HW573012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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