Anti-KMT5B Polyclonal Antibody (HA830014)

Name: Anti-KMT5B Polyclonal Antibody
Brand: abinScience
SKU: HA830014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HA830014

Description

Anti-KMT5B Polyclonal Antibody (HA830014) is a rabbit polyclonal antibody detecting Histone-lysine N-methyltransferase KMT5B; Lysine N-methyltransferase 5B; Lysine-specific methyltransferase 5B; Suppressor of variegation 4-20 homolog 1; Su(var)4-20 homolog 1; Suv4-20h1; [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B; 2.1.1.362; [histone H4]-lysine20 N-methyltransferase KMT5B; 2.1.1.361; KMT5B; SUV420H1; CGI-85 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human KMT5B (Met72-Ser368).

Target

Histone-lysine N-methyltransferase KMT5B; Lysine N-methyltransferase 5B; Lysine-specific methyltransferase 5B; Suppressor of variegation 4-20 homolog 1; Su(var)4-20 homolog 1; Suv4-20h1; [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B; 2.1.1.362; [histone H4]-lysine20 N-methyltransferase KMT5B; 2.1.1.361; KMT5B; SUV420H1; CGI-85

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q4FZB7, Q3U8K7, Q29RP8, P0C2N5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histone-lysine N-methyltransferase KMT5B is a ~99 kDa protein. Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).

1. Wu, H. et al. (2013) FEBS letters 587, 3859-68. PMID: 24396869

2. Bromberg, KD. et al. (2017) Nature chemical biology 13, 317-324. PMID: 28114273

3. Neguembor, MV. et al. (2013) Journal of molecular cell biology 5, 294-307. PMID: 23720823

Note

For research use only

Images

References

Datasheet

Anti-KMT5B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-KMT5B Polyclonal Antibody (HA830014)

Overview

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References

Datasheet

SDS

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