Anti-KPNA1 Polyclonal Antibody (HW602014)

Name: Anti-KPNA1 Polyclonal Antibody
Brand: abinScience
SKU: HW602014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW602014

Description

Anti-KPNA1 Polyclonal Antibody (HW602014) is a rabbit polyclonal antibody detecting KPNA1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human KPNA1 (Leu239-Thr506).

Target

KPNA1, SRP1-beta, Importin subunit alpha-5, RCH2, Karyopherin subunit alpha-1, Nucleoprotein interactor 1, RAG cohort protein 2, NPI-1

Purification

Purified by antigen affinity column.

Accession

P52294

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Importin subunit alpha-5 (KPNA1) is a ~60 kDa protein. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

1. Seo, HG. et al. (2016) Scientific reports 6, 34614. PMID: 27713473

2. Moroianu, J. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 2008-11. PMID: 7892216

3. Moroianu, J. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 6572-6. PMID: 8692858

4. Yang, TJ. et al. (2022) The Journal of cell biology 221. PMID: 35446349

Note

For research use only.

Images

References

Datasheet

Anti-KPNA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-KPNA1 Polyclonal Antibody (HW602014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us