Anti-LC3B Polyclonal Antibody (HV519014)

Name: Anti-LC3B Polyclonal Antibody
Brand: abinScience
SKU: HV519014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV519014

Description

Anti-LC3B Polyclonal Antibody (HV519014) is a rabbit polyclonal antibody detecting MAP1LC3B in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human LC3B (Met1-Gly120).

Target

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9GZQ8, A6NCE7, O41515, Q9CQV6, Q62625

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Microtubule-associated protein 1 light chain 3 beta (LC3B/MAP1LC3B) is a ~14 kDa protein. Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

1. Weidberg, H. et al. (2010) The EMBO journal 29, 1792-802. PMID: 20418806

2. Zhu, Y. et al. (2013) The Journal of biological chemistry 288, 1099-113. PMID: 23209295

3. Wei, Y. et al. (2017) Cell 168, 224-238.e10. PMID: 28017329

4. Sentelle, RD. et al. (2012) Nature chemical biology 8, 831-8. PMID: 22922758

5. Tang, Z. et al. (2013) Nature 502, 254-7. PMID: 24089205

6. An, H. et al. (2019) Molecular cell 74, 891-908.e10. PMID: 31006537

7. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538

Note

For research use only.

Images

References

Datasheet

Anti-LC3B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-LC3B Polyclonal Antibody (HV519014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us