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Anti-LDLRAP1 Polyclonal Antibody (HB294014)

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Overview
Catalog No.HB294014
Description
Anti-LDLRAP1 Polyclonal Antibody (HB294014) is a rabbit polyclonal antibody detecting Low density lipoprotein receptor adapter protein 1; Autosomal recessive hypercholesterolemia protein; LDLRAP1; ARH in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human LDLRAP1 (Glu33-Gly189).
Target Low density lipoprotein receptor adapter protein 1; Autosomal recessive hypercholesterolemia protein; LDLRAP1; ARH
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5SW96, Q8C142, D3ZAR1, Q801G1, Q67FQ3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Low density lipoprotein receptor adapter protein 1 (LDLRAP1) is a ~33 kDa protein. Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytosis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.

Note For research use only
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Formula
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-LDLRAP1 Polyclonal Antibody

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