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Anti-LIF Polyclonal Antibody (HB754014)

Anti-LIF Polyclonal Antibody
Anti-LIF Polyclonal Antibody
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  • 50ug
  • 100ug
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Overview
Catalog No.HB754014
Description
Anti-LIF Polyclonal Antibody (HB754014) is a rabbit polyclonal antibody detecting LIF in ELISA, IHC, WB. Suitable for Human, Bovine, and Neovison vison.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Bos taurus, Neovison vison
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human LIF (Pro24-Phe202).
Target Emfilermin, D factor, HILDA, LIF, MLPLI, Melanoma-derived LPL inhibitor, Leukemia inhibitory factor, Differentiation-stimulating factor
Purification Purified by antigen affinity column.
Accession P15018
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Leukemia inhibitory factor (LIF) is a ~22 kDa protein. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Note For research use only.
Images
  • Anti-LIF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LIF polyclonal antibody at 1ug/mL on recombinant LIF (Catalog No. HB754012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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