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Anti-LIG1 Polyclonal Antibody (HB281014)

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Overview
Catalog No.HB281014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human LIG1 (Gly484-Ile663).
Target DNA ligase 1, Polydeoxyribonucleotide synthase [ATP] 1, DNA ligase I, LIG1
Purification Purified by antigen affinity column.
Accession P18858
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA ligase 1 (LIG1) is a ~101 kDa protein. DNA ligase that seals nicks in double-stranded during DNA repair. Also involved in DNA replication and DNA recombination.

1. Maffucci, P. et al. (2018) The Journal of clinical investigation 128, 5489-5504. PMID: 30395541
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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