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Anti-LIMS1 Polyclonal Antibody (HW323014)

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Overview
Catalog No.HW323014
Description
Anti-LIMS1 Polyclonal Antibody (HW323014) is a rabbit polyclonal antibody detecting LIMS1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human LIMS1 (Ala68-Cys303).
Target PINCH, Renal carcinoma antigen NY-REN-48, PINCH1, LIM and senescent cell antigen-like-containing domain protein 1, LIMS1, PINCH-1, Particularly interesting new Cys-His protein 1
Purification Purified by antigen affinity column.
Accession P48059
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) is a ~37 kDa protein. Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-LIMS1 Polyclonal Antibody

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