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Anti-LIPG Polyclonal Antibody (HV170014)

Anti-LIPG Polyclonal Antibody
Anti-LIPG Polyclonal Antibody
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Overview
Catalog No.HV170014
Description
Anti-LIPG Polyclonal Antibody (HV170014) is a rabbit polyclonal antibody detecting LIPG in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human LIPG (Ala175-Ile412).
Target Phospholipase A1, Endothelial lipase, EDL, LIPG, EL, Endothelial cell-derived lipase
Purification Purified by antigen affinity column.
Accession Q9Y5X9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Endothelial lipase (LIPG) is a ~56 kDa protein. Exerts both phospholipase and triglyceride lipase activities. More active as a phospholipase than a triglyceride lipase. Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins.

1. Jaye, M. et al. (1999) Nature genetics 21, 424-8. PMID: 10192396
2. Hirata, K. et al. (1999) The Journal of biological chemistry 274, 14170-5. PMID: 10318835
3. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167
Note For research use only.
Images
  • Anti-LIPG Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LIPG polyclonal antibody at 1ug/mL on recombinant LIPG (Catalog No. HV170012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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