Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Recombinant Antibody (SAA0363) (JN937013)

Name: Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Recombinant Antibody (SAA0363)
Brand: abinScience
SKU: JN937013
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN937013

Description

Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Recombinant Antibody (SAA0363) (JN937013) is a human monoclonal antibody detecting InlB/Internalin B in ELISA, Neutralization, SPR. Suitable for Listeria monocytogenes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Listeria monocytogenes

Applications

ELISA, Neutralization, SPR

Host species

Human

Clonality

Monoclonal

Isotype

VHH-huFc

Clone ID

SAA0363

Target

Internalin B, InlB, Invasion protein InlB, inlB, lmo0434

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0DQD2, P0DQD3

RRID

Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Antibody (SAA0363) (abinScience Cat# JN937013, RRID:AB_3725906)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Internalin B (InlB) is a ~71 kDa protein. Mediates the entry of L.monocytogenes into normally non-phagocytic mammalian host cells (Probable). Its host receptor is hepatocyte growth factor receptor (HGF receptor, a tyrosine kinase, MET) which is tyrosine-phosphorylated in response to InlB in human, green monkey, mouse and dog cell lines. Downstream adapter proteins GAB1 and CBL are phosphorylated in response to InlB, which also causes cell colony scattering. InlB binding to mammalian cells is saturable and inhibited by EDTA; InlB-coated beads can be taken up by host cells. Complement component 1 Q subcomponent-binding protein (gC1q-R, C1QBP) might act as an InlB receptor, leading to activation of PI3-kinase in green monkey cells.

1. Shen, Y. et al. (2000) Cell 103, 501-10. PMID: 11081636

2. Braun, L. et al. (1997) Molecular microbiology 25, 285-94. PMID: 9282740

3. Banerjee, M. et al. (2004) Molecular microbiology 52, 257-71. PMID: 15049825

4. Braun, L. et al. (2000) The EMBO journal 19, 1458-66. PMID: 10747014

5. Marino, M. et al. (2002) The EMBO journal 21, 5623-34. PMID: 12411480

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Antibody (SAA0363).

References

Datasheet

Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Recombinant Antibody (SAA0363).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Listeria monocytogenes serovar 1/2a InlB/Internalin B Recombinant Antibody (SAA0363) (JN937013)

Overview

Images

References

Datasheet

SDS