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Anti-LRCH4 Polyclonal Antibody (HD427014)

Anti-LRCH4 Polyclonal Antibody
Anti-LRCH4 Polyclonal Antibody
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Overview
Catalog No.HD427014
Description
Anti-LRCH4 Polyclonal Antibody (HD427014) is a rabbit polyclonal antibody detecting LRCH4 in ELISA, IHC, WB. Suitable for Mouse and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human LRCH4 (Gly27-Glu373).
Target LRCH4, LRN, LRRN1, LRRN4, Leucine-rich neuronal protein, Leucine-rich repeat and calponin homology domain-containing protein 4, Leucine-rich repeat neuronal protein 4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O75427
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesO75427
Background

Leucine-rich repeat and calponin homology domain-containing protein 4 (LRCH4) is a ~73 kDa protein. Accessory protein that regulates signaling by multiple TLRs, acting as a broad-spanning regulator of the innate immune response. In macrophages, binds LPS and promotes proper docking of LPS in lipid raft membrane. May be required for lipid raft maintenance.

Note For research use only
Images
  • Anti-LRCH4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LRCH4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: SH-SY5Y cell lysate
    Lane 2: K562 cell lysate
    Lane 3: MCF-7 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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