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Anti-LY96 Polyclonal Antibody (HV554014)

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Overview
Catalog No.HV554014
Description
Anti-LY96 Polyclonal Antibody (HV554014) is a rabbit polyclonal antibody detecting LY96 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human LY96 (Glu17-Asn160).
Target Ly-96, ESOP-1, MD2, Lymphocyte antigen 96, Protein MD-2, LY96, ESOP1
Purification Purified by antigen affinity column.
Accession Q9Y6Y9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Lymphocyte antigen 96 (LY96) is a ~18 kDa protein. Binds bacterial lipopolysaccharide (LPS). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS.

1. Ohto, U. et al. (2007) Science (New York, N.Y.) 316, 1632-4. PMID: 17569869
2. Kim, HM. et al. (2007) Cell 130, 906-17. PMID: 17803912
3. Dziarski, R. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 1938-44. PMID: 11160242
4. Visintin, A. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 12156-61. PMID: 11593030
5. Shimazu, R. et al. (1999) The Journal of experimental medicine 189, 1777-82. PMID: 10359581
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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