Anti-MACROH2A1 Polyclonal Antibody (HF633014)

Name: Anti-MACROH2A1 Polyclonal Antibody
Brand: abinScience
SKU: HF633014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF633014

Description

Anti-MACROH2A1 Polyclonal Antibody (HF633014) is a rabbit polyclonal antibody detecting MACROH2A1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MACROH2A1 (Ile109-Leu369).

Target

Histone macroH2A1, mH2A1, H2A/y, MACROH2A1, Medulloblastoma antigen MU-MB-50.205, Core histone macro-H2A.1, H2AFY, Histone H2A.y

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O75367

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Core histone macro-H2A.1 (MACROH2A1) is a ~39 kDa protein. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation.

1. Angelov, D. et al. (2003) Molecular cell 11, 1033-41. PMID: 12718888

2. Zhang, R. et al. (2005) Developmental cell 8, 19-30. PMID: 15621527

3. Doyen, CM. et al. (2006) Molecular and cellular biology 26, 1156-64. PMID: 16428466

4. Hernández-Muñoz, I. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 7635-40. PMID: 15897469

5. Chakravarthy, S. et al. (2005) Molecular and cellular biology 25, 7616-24. PMID: 16107708

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MACROH1A2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: Hela cell lysate
Lane 3: Rat brain lysate
Lane 4: Rat liver lysate
Lane 5: 293T cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Rat lung lysate