Anti-MAP3K7 Polyclonal Antibody (HT050014)

Name: Anti-MAP3K7 Polyclonal Antibody
Brand: abinScience
SKU: HT050014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HT050014

Description

Anti-MAP3K7 Polyclonal Antibody (HT050014) is a rabbit polyclonal antibody detecting MAP3K7 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAP3K7 (Glu30-Thr285).

Target

Mitogen-activated protein kinase kinase kinase 7, TAK1, MAP3K7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O43318

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase kinase kinase 7 (MAP3K7) is a ~67 kDa protein. Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs); both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1).

1. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. PMID: 10094049

2. Wang, C. et al. (2001) Nature 412, 346-51. PMID: 11460167

3. Edlund, S. et al. (2003) Molecular biology of the cell 14, 529-44. PMID: 12589052

4. Thiefes, A. et al. (2006) EMBO reports 7, 838-44. PMID: 16845370

5. Huangfu, WC. et al. (2006) The Journal of biological chemistry 281, 28802-10. PMID: 16893890

6. Pertel, T. et al. (2011) Nature 472, 361-5. PMID: 21512573

7. Moriguchi, T. et al. (1996) The Journal of biological chemistry 271, 13675-9. PMID: 8663074

8. Shirakabe, K. et al. (1997) The Journal of biological chemistry 272, 8141-4. PMID: 9079627

9. Ma, M. et al. (2023) Molecular cell 83, 3885-3903.e5. PMID: 37832545

Note

For research use only.

Images

References

Datasheet

Anti-MAP3K7 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-MAP3K7 Polyclonal Antibody (HT050014)

Overview

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References

Datasheet

SDS

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