Anti-MAPK14 Polyclonal Antibody (HS804014)

Name: Anti-MAPK14 Polyclonal Antibody
Brand: abinScience
SKU: HS804014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HS804014

Description

Anti-MAPK14 Polyclonal Antibody (HS804014) is a rabbit polyclonal antibody detecting MAPK14 in ELISA, IHC, WB. Suitable for Dog, Cyprinus carpio, Danio rerio, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPK14 (Met1-Ser360).

Target

Stress-activated protein kinase 2a, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, SAPK2A, Cytokine suppressive anti-inflammatory drug-binding protein, CSBP, CSPB1, MAP kinase 14, MAP kinase MXI2, MAPK14, CSBP2, MAP kinase p38 alpha, MAPK 14, MXI2, CSAID-binding protein, MAX-interacting protein 2, CSBP1, SAPK2a

Purification

Purified by antigen affinity column.

Accession

Q16539

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase 14 (MAPK14) is a ~41 kDa protein. Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1.

1. Deak, M. et al. (1998) The EMBO journal 17, 4426-41. PMID: 9687510

2. Pierrat, B. et al. (1998) The Journal of biological chemistry 273, 29661-71. PMID: 9792677

3. Scheper, GC. et al. (2001) Molecular and cellular biology 21, 743-54. PMID: 11154262

4. Sayed, M. et al. (2000) The Journal of biological chemistry 275, 16569-73. PMID: 10747897

5. Khurana, A. et al. (2006) The Journal of biological chemistry 281, 35316-26. PMID: 17003045

6. Webber, JL. et al. (2010) The EMBO journal 29, 27-40. PMID: 19893488

7. Zwang, Y. et al. (2006) The EMBO journal 25, 4195-206. PMID: 16932740

8. Xu, P. et al. (2010) Molecular cell 37, 551-66. PMID: 20188673

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAPK14 polyclonal antibody at 1ug/mL on recombinant MAPK14 (Catalog No. HS804012).

References

Datasheet

Anti-MAPK14 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-MAPK14 Polyclonal Antibody (HS804014)

Overview

Images

References

Datasheet

SDS

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