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Anti-MARK1 Polyclonal Antibody (HB600014)

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Overview
Catalog No.HB600014
Description
Anti-MARK1 Polyclonal Antibody (HB600014) is a rabbit polyclonal antibody detecting Serine/threonine-protein kinase MARK1; 2.7.11.1; 2.7.11.26; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c; MARK1; KIAA1477; MARK in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human MARK1 (Gly58-Lys371).
Target Serine/threonine-protein kinase MARK1; 2.7.11.1; 2.7.11.26; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c; MARK1; KIAA1477; MARK
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9P0L2, O08678, Q8VHJ5, Q8VHF0, Q03141, P27448, Q7KZI7, O08679, Q05512
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Serine/threonine-protein kinase MARK1 is a ~89 kDa protein. Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly.

1. Gu, GJ. et al. (2013) Neuromolecular medicine 15, 458-69. PMID: 23666762
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-MARK1 Polyclonal Antibody

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