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Anti-MARV VP30/Minor nucleoprotein VP30 Polyclonal Antibody (VK538014)

Anti-MARV VP30/Minor nucleoprotein VP30 Polyclonal Antibody
Anti-MARV VP30/Minor nucleoprotein VP30 Polyclonal Antibody
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Overview
Catalog No.VK538014
Description
Anti-MARV VP30/Minor nucleoprotein VP30 Polyclonal Antibody (VK538014) is a rabbit polyclonal antibody detecting Minor nucleoprotein VP30 in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityLake Victoria marburgvirus (MARV) (Marburg virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant MARV VP30/Minor nucleoprotein VP30 (Lys146-Gln281).
Target Transcriptional activator VP30, Minor nucleoprotein VP30, VP30
Purification Purified by antigen affinity column.
Accession P35258
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Transcriptional activator VP30 (Minor nucleoprotein VP30) is a ~31 kDa protein. Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA secondary structure at the transcription start site of the first gene.

Note For research use only.
Images
  • Anti-MARV VP30/Minor nucleoprotein VP30 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MARV VP30/Minor nucleoprotein VP30 polyclonal antibody at 1ug/mL on recombinant MARV VP30/Minor nucleoprotein VP30 (Catalog No. VK538012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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