Anti-Matrix Protein Polyclonal Antibody (VK499034)

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Overview

Catalog No.

VK499034

Description

Anti-Matrix Protein Polyclonal Antibody (VK499034) is a rabbit polyclonal antibody detecting matrix protein; M in ELISA, IHC, WB. Suitable for Nipah virus and Hendra virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Nipah virus, Hendra virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant nipah virus Matrix (Met1-Gly352).

Target

matrix protein; M

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9IK90, O89341

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Matrix protein is a ~39 kDa protein. Plays a crucial role in virion assembly and budding. Forms a shell at the inner face of the plasma membrane. Transits through the host nucleus before gaining the functional ability to localize and bud from the plasma membrane. Mediates together with fusion protein the incorporation of the glycoprotein to the viral particles. Also participates in the inhibition of the host interferon type I antiviral response by interacting with and thereby inhibiting host TRIM6.

1. Ciancanelli, MJ. et al. (2006) Journal of virology 80, 12070-8. PMID: 17005661

2. Patch, JR. et al. (2007) Virology journal 4, 1. PMID: 17204159

3. Patch, JR. et al. (2008) Virology journal 5, 137. PMID: 19000317

4. Wang, YE. et al. (2010) PLoS pathogens 6, e1001186. PMID: 21085610

5. Johnston, GP. et al. (2017) Journal of virology 91. PMID: 28250132

6. Bharaj, P. et al. (2016) PLoS pathogens 12, e1005880. PMID: 27622505

Note

For research use only

Images

References

Datasheet

Anti-Matrix Protein Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Matrix Protein Polyclonal Antibody (VK499034)

Overview

Images

References

Datasheet

SDS

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