Anti-MB Polyclonal Antibody (HF785014)

Name: Anti-MB Polyclonal Antibody
Brand: abinScience
SKU: HF785014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF785014

Description

Anti-MB Polyclonal Antibody (HF785014) is a rabbit polyclonal antibody detecting MB in ELISA, IHC, WB. Suitable for Human, Aotus trivirgatus, Balaena mysticetus, Balaenoptera edeni, and Callithrix jacchus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human MB (Met1-Gly154).

Target

Myoglobin, MB

Purification

Purified by antigen affinity column.

Accession

P02144

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Myoglobin (MB) is a ~17 kDa protein. Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity.

1. Olivé, M. et al. (2019) Nature communications 10, 1396. PMID: 30918256

2. Hofbauer, S. et al. (2022) The FEBS journal 289, 1105-1117. PMID: 34679218

3. Quesnelle, K. et al. (2020) Nitric oxide : biology and chemistry 104-105, 36-43. PMID: 32891753

Note

For research use only.

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References

Datasheet