Anti-MDH1 Polyclonal Antibody (HW769014)

Name: Anti-MDH1 Polyclonal Antibody
Brand: abinScience
SKU: HW769014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW769014

Description

Anti-MDH1 Polyclonal Antibody (HW769014) is a rabbit polyclonal antibody detecting MDH1 in ELISA, IHC, WB. Suitable for Bovine, Cat, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MDH1 (Ser2-Ala334).

Target

MDHA, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, MDH1, Diiodophenylpyruvate reductase

Purification

Purified by antigen affinity column.

Accession

P40925

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Malate dehydrogenase, cytoplasmic (MDH1) is a ~36 kDa protein. Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS).

1. Friedrich, CA. et al. (1987) Biochemical genetics 25, 657-69. PMID: 2449162

2. Friedrich, CA. et al. (1988) Annals of human genetics 52, 25-37. PMID: 3052244

3. Broeks, MH. et al. (2019) Human genetics 138, 1247-1257. PMID: 31538237

4. Zhang, JY. et al. (2021) Cell research 31, 980-997. PMID: 34012073

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MDH1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: THP-1 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate
Lane11: Rat liver lysate
Lane12: Rat kidney lysate

References

Datasheet

Anti-MDH1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MDH1 Polyclonal Antibody (HW769014)

Overview

Images

References

Datasheet

SDS

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