Anti-MERS-CoV ORF4b Polyclonal Antibody (VK073034)

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Overview

Catalog No.

VK073034

Description

Anti-MERS-CoV ORF4b Polyclonal Antibody (VK073034) is a rabbit polyclonal antibody detecting Non-structural protein ORF4b; ORF4b; ORF4b in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Middle East respiratory syndrome-related coronavirus (MERS-CoV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant mers-cov ORF4b (Met1-Phe246(Pro44His)).

Target

Non-structural protein ORF4b; ORF4b; ORF4b

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

K9N643

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Non-structural protein ORF4b is a ~28 kDa protein. Plays a role in the inhibition of host innate immunity by inhibiting the interaction between host IKBKE and MAVS. In turn, this inhibition prevents the production of host interferon beta. Additionally, inhibits host NF-kappa-B by interacting with host KPNA4 and thereby preventing the translocation of the NF-kappa-B complex into the nucleus by this importin.

1. Canton, J. et al. (2018) PLoS pathogens 14, e1006838. PMID: 29370303

Note

For research use only

Images

References

Datasheet

Anti-MERS-CoV ORF4b Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MERS-CoV ORF4b Polyclonal Antibody (VK073034)

Overview

Images

References

Datasheet

SDS

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