Anti-MeV Nucleoprotein Polyclonal Antibody (VK397014)

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Overview

Catalog No.

VK397014

Description

Anti-MeV Nucleoprotein Polyclonal Antibody (VK397014) is a rabbit polyclonal antibody detecting Nucleoprotein. Suitable for Measles virus.

Species reactivity

Measles virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant MeV Nucleoprotein (Thr21-Arg408).

Target

N, NP, Nucleocapsid protein, Nucleoprotein, Protein N

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q77M43

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P.

1. Desfosses, A. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 4256-4264. PMID: 30787192

2. Zhou, Y. et al. (2019) Journal of virology 93. PMID: 31375591

Note

For research use only

Images

References

Datasheet

Anti-MeV Nucleoprotein Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MeV Nucleoprotein Polyclonal Antibody (VK397014)

Overview

Images

References

Datasheet

SDS

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