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Anti-MGAM/MGA Polyclonal Antibody (HT381014)

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Overview
Catalog No.HT381014
Description
Anti-MGAM/MGA Polyclonal Antibody (HT381014) is a rabbit polyclonal antibody detecting MGAM in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MGAM/MGA (Gly368-His731).
Target Maltase-glucoamylase, intestinal, Glucan 1, 4-alpha-glucosidase, MGA, Alpha-glucosidase, MGAML, MGAM
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43451
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Maltase-glucoamylase (MGAM/MGA) is a ~312 kDa protein. Alpha-(1,4) exo-glucosidase involved in breakdown of dietary starch oligosaccharides in small intestine. Cleaves the non-reducing alpha-(1,4)-linked glucose residue in linear dextrins with retention of anomeric center stereochemistry. Mainly hydrolyzes short length oligomaltoses having two to seven glucose residues. Can cleave alpha-(1,2), alpha-(1,3) and alpha-(1,6) glycosidic linkages with lower efficiency, whereas beta glycosidic linkages are usually not hydrolyzed.

1. Nichols, BL. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1432-7. PMID: 12547908
2. Sim, L. et al. (2008) Journal of molecular biology 375, 782-92. PMID: 18036614
3. Quezada-Calvillo, R. et al. (2008) The Journal of nutrition 138, 685-92. PMID: 18356321
4. Ren, L. et al. (2011) Protein & cell 2, 827-36. PMID: 22058037
5. Lee, BH. et al. (2016) Journal of agricultural and food chemistry 64, 6487-94. PMID: 27480812
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-MGAM/MGA Polyclonal Antibody

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