Anti-MGAT3 Polyclonal Antibody (HB485014)

Name: Anti-MGAT3 Polyclonal Antibody
Brand: abinScience
SKU: HB485014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HB485014

Description

Anti-MGAT3 Polyclonal Antibody (HB485014) is a rabbit polyclonal antibody detecting 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; 2.4.1.144; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; GNT-III; GlcNAc-T III; N-acetylglucosaminyltransferase III; MGAT3; GGNT3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human MGAT3 (Ser87-Val533).

Target

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; 2.4.1.144; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; GNT-III; GlcNAc-T III; N-acetylglucosaminyltransferase III; MGAT3; GGNT3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q09327, Q10470, Q02527

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (MGAT3) is a ~61 kDa protein. It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains, called bisecting N-acetylglucosamine (GlcNAc). It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The addition of this bisecting GlcNAc residue alters not only the composition, but also the conformation of the N-glycan. The introduction of the bisecting GlcNAc residue results in the suppression of further processing and elongation of N-glycans, precluding the formation of beta-1,6 GlcNAc branching, catalyzed by MGAT5 since it is unable to use the bisected oligosaccharide as a substrate.

1. Pinho, SS. et al. (2009) Human molecular genetics 18, 2599-608. PMID: 19403558

2. Lu, J. et al. (2016) The Journal of biological chemistry 291, 5708-5720. PMID: 26801611

Note

For research use only

Images

References

Datasheet

Anti-MGAT3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-MGAT3 Polyclonal Antibody (HB485014)

Overview

Images

References

Datasheet

SDS

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