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Anti-Mgp3 Polyclonal Antibody (JN128014)

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Overview
Catalog No.JN128014
Description
Anti-Mgp3 Polyclonal Antibody (JN128014) is a rabbit polyclonal antibody detecting Mgp-operon protein 3; Mgp3; ORF-3 protein; MG192 in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityMycoplasma genitalium
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mgp3 (Asn26-Asp408).
Target Mgp-operon protein 3; Mgp3; ORF-3 protein; MG192
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P22747
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adhesin P110 (Mgp3) is a ~114 kDa protein. Part of the major adhesin Nap complex which mediates attachment to host ciliated epithelium. This protein and P140 (mgpB) mutually stabilize each other, and are required for formation of the attachment organelle. Binds sialylated oligosaccharides, which are present on the surface of its human host cells and which probably serve as the main host cell receptors for this bacterium. The Nap complex plays a major role in gliding motility and adhesion to host cells.

1. Burgos, R. et al. (2006) Journal of bacteriology 188, 8627-37. PMID: 17028283
2. Aparicio, D. et al. (2018) Nature communications 9, 4471. PMID: 30367053
3. Marseglia, A. et al. (2024) International journal of biological macromolecules 279, 135277. PMID: 39226978
4. García-Morales, L. et al. (2016) Molecular microbiology 100, 125-38. PMID: 26712501
5. Seybert, A. et al. (2018) Molecular microbiology 108, 319-329. PMID: 29470847
6. Aparicio, D. et al. (2020) Nature communications 11, 2877. PMID: 32513917
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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