Anti-MIB1 Polyclonal Antibody (HV353014)

Name: Anti-MIB1 Polyclonal Antibody
Brand: abinScience
SKU: HV353014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HV353014

Description

Anti-MIB1 Polyclonal Antibody (HV353014) is a rabbit polyclonal antibody detecting MIB1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MIB1 (Arg769-Glu1000).

Target

Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP-1, Mind bomb homolog 1, ZZANK2, DAPK-interacting protein 1, E3 ubiquitin-protein ligase MIB1, KIAA1323, DIP1, RING-type E3 ubiquitin transferase MIB1

Purification

Purified by antigen affinity column.

Accession

Q86YT6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase MIB1 is a ~110 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

Note

For research use only.

Images

References

Datasheet

Anti-MIB1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MIB1 Polyclonal Antibody (HV353014)

Overview

Images

References

Datasheet

SDS

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