Anti-MLLT3 Polyclonal Antibody (HA633014)

Name: Anti-MLLT3 Polyclonal Antibody
Brand: abinScience
SKU: HA633014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA633014

Description

Anti-MLLT3 Polyclonal Antibody (HA633014) is a rabbit polyclonal antibody detecting Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human MLLT3 (Met1-Thr148).

Target

Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P42568, A2AM29

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein AF-9 (MLLT3) is a ~63 kDa protein. Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3.

1. Lin, C. et al. (2010) Molecular cell 37, 429-37. PMID: 20159561

2. He, N. et al. (2010) Molecular cell 38, 428-38. PMID: 20471948

3. Li, Y. et al. (2014) Cell 159, 558-71. PMID: 25417107

4. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114

5. Zhang, Q. et al. (2016) Structure (London, England : 1993) 24, 1606-12. PMID: 27545619

6. Li, X. et al. (2018) Nature chemical biology 14, 1140-1149. PMID: 30374167

7. Klein, BJ. et al. (2018) Nature communications 9, 4574. PMID: 30385749

9. Becht, DC. et al. (2025) Nature structural & molecular biology 32, 709-718. PMID: 39794553

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-AF9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate

References

Datasheet

Anti-MLLT3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-MLLT3 Polyclonal Antibody (HA633014)

Overview

Images

References

Datasheet

SDS