Anti-MMP13 Polyclonal Antibody (HW650014)

Name: Anti-MMP13 Polyclonal Antibody
Brand: abinScience
SKU: HW650014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HW650014

Description

Anti-MMP13 Polyclonal Antibody (HW650014) is a rabbit polyclonal antibody detecting MMP13 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Equus caballus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MMP13 (Leu20-Cys471).

Target

MMP-13, MMP13, Matrix metalloproteinase-13, Collagenase 3

Purification

Purified by antigen affinity column.

Accession

P45452

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Collagenase 3 (MMP13) is a ~53 kDa protein. Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MMP13 polyclonal antibody at 1ug/mL on recombinant MMP13 (Catalog No. HW650012).

References

Datasheet

Anti-MMP13 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MMP13 Polyclonal Antibody (HW650014)

Overview

Images

References

Datasheet

SDS

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