Anti-MoMLV Reverse Transcriptase/RT Polyclonal Antibody (VK545014)

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Datasheet

Overview

Catalog No.

VK545014

Description

Anti-MoMLV Reverse Transcriptase/RT Polyclonal Antibody (VK545014) is a rabbit polyclonal antibody detecting Reverse transcriptase/ribonuclease H. Suitable for Radiation murine leukemia virus, Friend murine leukemia virus, Xenotropic MuLV-related virus, Murine leukemia virus, AKV murine leukemia virus, Cas-Br-E murine leukemia virus, and Moloney murine leukemia virus.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Radiation murine leukemia virus, Friend murine leukemia virus, Xenotropic MuLV-related virus, Murine leukemia virus, AKV murine leukemia virus, Cas-Br-E murine leukemia virus, Moloney murine leukemia virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant MoMLV Reverse Transcriptase/RT (Thr660-Pro1336).

Target

CA, Capsid protein p30, EC:2.7.7.-, EC:2.7.7.49, EC:2.7.7.7, EC:3.1.-.-, EC:3.1.26.4, EC:3.4.23.-, Gag-Pol polyprotein, IN, Integrase, MA, Matrix protein p15, NC-pol, Nucleocapsid protein p10-Pol, PR, Pr180gag-pol, Protease, RNA-binding phosphoprotein p12, RT, Reverse transcriptase/ribonuclease H, gag-pol, p14, p46, p80, pp12

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P03355

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Gag-Pol polyprotein (Reverse Transcriptase/RT) is a ~194 kDa protein. Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag-Pol, or to Gag-Pol binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.

Note

For research use only

Images

References

Datasheet

Anti-MoMLV Reverse Transcriptase/RT Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MoMLV Reverse Transcriptase/RT Polyclonal Antibody (VK545014)

Overview

Images

References

Datasheet

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