Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014)

Name: Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody
Brand: abinScience
SKU: MF562014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MF562014

Description

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014) is a rabbit polyclonal antibody detecting Adenosine deaminase in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse ADA/Adenosine deaminase (Met1-Gln352).

Target

Adenosine deaminase, 3.5.4.4, Adenosine aminohydrolase, Ada

Purification

Purified by antigen affinity column.

Accession

P03958

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Adenosine deaminase (ADA) is a ~39 kDa protein. Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.

1. Xu, PA. et al. (2000) Biochemical and biophysical research communications 269, 749-57. PMID: 10720488

2. Mohamedali, KA. et al. (1996) Biochemistry 35, 1672-80. PMID: 8634299

3. Sideraki, V. et al. (1996) Biochemistry 35, 7862-72. PMID: 8672487

4. Sideraki, V. et al. (1996) Biochemistry 35, 15019-28. PMID: 8942668

5. Blackburn, MR. et al. (1997) Development (Cambridge, England) 124, 3089-97. PMID: 9272950

6. Apasov, SG. et al. (2001) The Journal of clinical investigation 108, 131-41. PMID: 11435465

7. Li, G. et al. (2015) Pharmacology research & perspectives 3, e00121. PMID: 26038697

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ADA polyclonal antibody at ADA on various samples.
Lane 1: Jurkat cell lysate
Lane 2: MOLT-4 cell lysate
Lane 3: Mouse thymus lysate
Lane 4: Mouse stomach lysate
Lane 5: Rat spleen lysate

References

Datasheet

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse ADA/Adenosine deaminase Polyclonal Antibody (MF562014)

Overview

Images

References

Datasheet

SDS

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