Anti-Mouse AIM2 Polyclonal Antibody (MT230014)

Name: Anti-Mouse AIM2 Polyclonal Antibody
Brand: abinScience
SKU: MT230014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

MT230014

Description

Anti-Mouse AIM2 Polyclonal Antibody (MT230014) is a rabbit polyclonal antibody detecting Aim2 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse AIM2 (Glu153-Lys342).

Target

Interferon-inducible protein AIM2, Interferon-inducible protein 210, Ifi-210, Interferon-inducible protein p210, Aim2, Gm1313, Ifi210

Purification

Purified by antigen affinity column.

Accession

Q91VJ1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interferon-inducible protein AIM2 is a ~40 kDa protein. Sensor component of the AIM2 inflammasome, which mediates inflammasome activation in response to the presence of double-stranded DNA (dsDNA) in the cytosol, leading to subsequent pyroptosis. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): specifically recognizes and binds dsDNA in the cytosol, and mediates the formation of the inflammasome polymeric complex composed of AIM2, CASP1 and PYCARD/ASC. Recruitment of pro-caspase-1 (proCASP1) to the AIM2 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion. In some cells, CASP1 activation mediates cleavage and activation of GSDMD, triggering pyroptosis without promoting cytokine secretion.

1. Roberts, TL. et al. (2009) Science (New York, N.Y.) 323, 1057-60. PMID: 19131592

2. Hornung, V. et al. (2009) Nature 458, 514-8. PMID: 19158675

3. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13. PMID: 19158676

4. Bürckstümmer, T. et al. (2009) Nature immunology 10, 266-72. PMID: 19158679

5. Rathinam, VA. et al. (2010) Nature immunology 11, 395-402. PMID: 20351692

6. Fernandes-Alnemri, T. et al. (2010) Nature immunology 11, 385-93. PMID: 20351693

7. Sauer, JD. et al. (2010) Cell host & microbe 7, 412-9. PMID: 20417169

8. Jones, JW. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 9771-6. PMID: 20457908

9. Belhocine, K. et al. (2012) Cellular microbiology 14, 71-80. PMID: 21902795

10. Ru, H. et al. (2013) Cell research 23, 855-8. PMID: 23567559

Note

For research use only.

Images

References

Datasheet

Anti-Mouse AIM2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Mouse AIM2 Polyclonal Antibody (MT230014)

Overview

Images

References

Datasheet

SDS

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