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Anti-Mouse ANXA1 Polyclonal Antibody (MY216014)

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Anti-Mouse ANXA1 Polyclonal Antibody
Anti-Mouse ANXA1 Polyclonal Antibody
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Overview
Catalog No.MY216014
Description
Anti-Mouse ANXA1 Polyclonal Antibody (MY216014) is a rabbit polyclonal antibody detecting Anxa1. Suitable for Rat and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse ANXA1 (Ala2-Asn346).
Target Annexin A1, Annexin Ac2-26, Annexin I, Annexin-1, Anx1, Anxa1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Lpc-1, Lpc1, Phospholipase A2 inhibitory protein, p35
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P10107
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Annexin A1 (ANXA1) is a ~38 kDa protein. Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T cells. Promotes the differentiation of T cells into Th1 cells and negatively regulates differentiation into Th2 cells.

1. Hannon, R. et al. (2003) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17, 253-5. PMID: 12475898
2. D'Acquisto, F. et al. (2007) European journal of immunology 37, 3131-42. PMID: 17948261
3. Patel, DM. et al. (2011) Journal of cell science 124, 578-88. PMID: 21245195
Note For research use only
Images
  • Anti-Mouse ANXA1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ANXA1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: A549 cell lysate
    Lane 5: A431 cell lysate
    Lane 6: K562 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse ANXA1 Polyclonal Antibody

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