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Anti-Mouse ATP6V0D2 Polyclonal Antibody (MD459014)

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Overview
Catalog No.MD459014
Description
Anti-Mouse ATP6V0D2 Polyclonal Antibody (MD459014) is a rabbit polyclonal antibody detecting ATP6V0D2. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse ATP6V0D2 (Met1-Leu350).
Target ATP6V0D2, V-ATPase subunit d 2, V-type proton ATPase subunit d 2, Vacuolar proton pump subunit d 2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q80SY3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

V-type proton ATPase subunit d 2 (ATP6V0D2) is a ~40 kDa protein. Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. May play a role in coupling of proton transport and ATP hydrolysis. Regulator of osteoclast fusion and bone formation.

1. Nishi, T. et al. (2003) The Journal of biological chemistry 278, 46396-402. PMID: 12963731
2. Lee, SH. et al. (2006) Nature medicine 12, 1403-9. PMID: 17128270
Note For research use only
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse ATP6V0D2 Polyclonal Antibody

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