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Anti-Mouse C3AR1 Polyclonal Antibody (MS803014)

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Overview
Catalog No.MS803014
Description
Anti-Mouse C3AR1 Polyclonal Antibody (MS803014) is a rabbit polyclonal antibody detecting C3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; Complement component 3a receptor 1; C3ar1; C3r1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mouse C3AR1 (Arg161-Arg333).
Target C3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; Complement component 3a receptor 1; C3ar1; C3r1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O09047
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

C3a anaphylatoxin chemotactic receptor (C3AR1) is a ~53 kDa protein. Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a, stimulating chemotaxis, granule enzyme release and superoxide anion production. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. C3AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase.

1. Tornetta, MA. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 158, 5277-82. PMID: 9164946
2. Hsu, MH. et al. (1997) Immunogenetics 47, 64-72. PMID: 9382922
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse C3AR1 Polyclonal Antibody

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