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Anti-Mouse C4B Polyclonal Antibody (MY567014)

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Anti-Mouse C4B Polyclonal Antibody
Anti-Mouse C4B Polyclonal Antibody
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Overview
Catalog No.MY567014
Description
Anti-Mouse C4B Polyclonal Antibody (MY567014) is a rabbit polyclonal antibody detecting C4b in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse C4B (Glu988-Thr1317).
Target Complement C4-B, Complement C4 beta chain, Complement C4 alpha chain, C4a anaphylatoxin, Complement C4 gamma chain, C4b, C4
Purification Purified by antigen affinity column.
Accession P01029
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Complement C4-B (C4B) is a ~192 kDa protein. Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.

Note For research use only.
Images
  • Anti-Mouse C4B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-C4b polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse C4B Polyclonal Antibody

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