Anti-Mouse CASP8 Polyclonal Antibody (MC266014)

Name: Anti-Mouse CASP8 Polyclonal Antibody
Brand: abinScience
SKU: MC266014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MC266014

Description

Anti-Mouse CASP8 Polyclonal Antibody (MC266014) is a rabbit polyclonal antibody detecting Casp8 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CASP8 (Gly236-Pro480).

Target

Caspase-8, CASP-8, 3.4.22.61, Caspase-8 subunit p18, Caspase-8 subunit p10, Casp8

Purification

Purified by antigen affinity column.

Accession

O89110

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Benchoua, A. et al. (2002) The Journal of biological chemistry 277, 34217-22. PMID: 12065591

2. Sun, H. et al. (2008) Cell 133, 415-26. PMID: 18455983

3. Orning, P. et al. (2018) Science (New York, N.Y.) 362, 1064-1069. PMID: 30361383

4. Sarhan, J. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E10888-E10897. PMID: 30381458

5. Newton, K. et al. (2019) Nature 574, 428-431. PMID: 31511692

6. Fritsch, M. et al. (2019) Nature 575, 683-687. PMID: 31748744

7. Muendlein, HI. et al. (2021) Nature communications 12, 86. PMID: 33397971

8. Luo, X. et al. (1998) Cell 94, 481-90. PMID: 9727491

9. Li, H. et al. (1998) Cell 94, 491-501. PMID: 9727492

10. Rickard, JA. et al. (2014) Cell 157, 1175-88. PMID: 24813849

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CASP8 polyclonal antibody at CASP8 on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: HCT116 cell lysate
Lane 4: THP-1 cell lysate

References

Datasheet

Anti-Mouse CASP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CASP8 Polyclonal Antibody (MC266014)

Overview

Images

References

Datasheet

SDS

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