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Anti-Mouse EMMPRIN/CD147 Polyclonal Antibody (MW664014)

Anti-Mouse EMMPRIN/CD147 Polyclonal Antibody
Anti-Mouse EMMPRIN/CD147 Polyclonal Antibody
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Overview
Catalog No.MW664014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse CD147/BSG (Ala22-Arg325).
Tested applications WB
Target Basigin (Basic immunoglobulin superfamily) (HT7 antigen) (Membrane glycoprotein gp42) (CD antigen CD147)
Purification Purified by antigen affinity column.
Accession P18572
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Basigin (CD147/BSG) is a ~42 kDa protein. Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors.

1. Hori, K. et al. (2000) Investigative ophthalmology & visual science 41, 3128-33. PMID: 10967074
2. Ochrietor, JD. et al. (2001) Experimental eye research 72, 467-77. PMID: 11273674
3. Ochrietor, JD. et al. (2002) Vision research 42, 447-53. PMID: 11853760
4. Aït-Ali, N. et al. (2015) Cell 161, 817-32. PMID: 25957687
Note For research use only.
Images
  • Anti-Mouse EMMPRIN/CD147 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-BSG polyclonal antibody at 1ug/mL on various samples.
    Lane 1: U87 cell lysate
    Lane 2: Mouse spleen lysate
    Lane 3: Mouse heart lysate
    Lane 4: Mouse liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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