Anti-Mouse CD160 Polyclonal Antibody (MF985014)

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Overview

Catalog No.

MF985014

Description

Anti-Mouse CD160 Polyclonal Antibody (MF985014) is a rabbit polyclonal antibody detecting CD160 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD160 (Ile30-Gln146).

Target

Natural killer cell receptor BY55, CD160 antigen, CD160, BY55

Purification

Purified by antigen affinity column.

Accession

O88875

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CD160 antigen is a ~20 kDa protein. Receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells. Receptor for both classical and non-classical MHC class I molecules. Receptor or ligand for TNF superfamily member TNFRSF14, participating in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. Upon ligation of TNFRSF14, provides stimulatory signal to NK cells enhancing IFNG production and anti-tumor immune response.

1. Maeda, M. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 4426-32. PMID: 16177084

2. Tu, TC. et al. (2015) The Journal of experimental medicine 212, 415-29. PMID: 25711213

3. Shui, JW. et al. (2012) Nature 488, 222-5. PMID: 22801499

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Mouse CD160 polyclonal antibody at 1ug/mL on recombinant Mouse CD160 (Catalog No. MF985012).
Western blot

Lane 1: Rat kidney lysate

References

Datasheet

Anti-Mouse CD160 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD160 Polyclonal Antibody (MF985014)

Overview

Images

References

Datasheet

SDS

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