Anti-Mouse CD19 Recombinant Antibody (1D3), APC (MB996137)

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Catalog No.	MB996137
Description	Anti-Mouse CD19 Antibody (1D3), APC [1D3] (MB996137) is a rat monoclonal antibody detecting CD19 in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Mouse
Applications	FCM
Host species	Rat
Isotype	IgG2a, kappa
Clone ID	1D3
Conjugation	APC
Clonality	Monoclonal
Target	B-lymphocyte antigen CD19, Differentiation antigen CD19, CD19
Endotoxin level	Please contact with the lab for this information.
Accession	P25918
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	B-lymphocyte antigen CD19 is a ~60 kDa protein. Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. CD19 is the therapeutic target of tafasitamab (Monjuvi). 1. Wang, Y. et al. (2002) Immunity 17, 501-14. PMID: 12387743 2. Ishiura, N. et al. (2010) European journal of immunology 40, 1192-204. PMID: 20101619 3. Buhl, AM. et al. (1997) The Journal of experimental medicine 186, 1897-910. PMID: 9382888 4. Engel, P. et al. (1995) Immunity 3, 39-50. PMID: 7542548 5. Rickert, RC. et al. (1995) Nature 376, 352-5. PMID: 7543183 6. Sato, S. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 159, 3278-87. PMID: 9317126
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-Mouse CD19 antibody. Mouse spleen cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Mouse CD19 antibody monoclonal antibody (Catalog # MB996137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Mouse CD19 Recombinant Antibody (1D3), APC.pdf

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Anti-Mouse CD19 Recombinant Antibody (1D3), APC (MB996137)

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