Anti-Mouse CD198/CCR8 Polyclonal Antibody (MW444014)

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Datasheet

Overview

Catalog No.

MW444014

Description

Anti-Mouse CD198/CCR8 Polyclonal Antibody (MW444014) is a rabbit polyclonal antibody detecting C-C chemokine receptor type 8; C-C CKR-8; CC-CKR-8; CCR-8; CDw198; Ccr8; Cmkbr8; Ter1 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant mouse CD198/CCR8 (Asp2-Met33&Asn92-Lys105&Tyr170-Ile200&His262-Leu278).

Target

C-C chemokine receptor type 8; C-C CKR-8; CC-CKR-8; CCR-8; CDw198; Ccr8; Cmkbr8; Ter1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P56484

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

C-C chemokine receptor type 8 (CD198/CCR8) is a ~40 kDa protein. G protein-coupled receptor that can bind a variety of chemokines, such as CCL1, CCL8, CCL16, CCL18. Regulates monocyte and eosinophil chemotaxis. Undergoes internalization upon CCL18 binding, leading to induced migration and calcium flux of highly polarized Th2 cells. In microglial cells, promotes phagocytosis with CCL18 through NF-kappa-B and Src signaling pathways. Stimulation of the CCL1-CCR8 signaling axis protects the gut from acute intestinal damage by supporting lymphoid cells IFN-gamma production.

1. Goya, I. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 1975-81. PMID: 9469461

2. Chensue, SW. et al. (2001) The Journal of experimental medicine 193, 573-84. PMID: 11238588

3. Kang, L. et al. (2020) Frontiers in immunology 11, 609400. PMID: 33613532

Note

For research use only

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References

Datasheet